Aliases:	CRYAB; Crystallin Alpha B; HSPB5; CRYA2; Renal Carcinoma Antigen NY-REN-27; Heat Shock Protein Beta-5; Rosenthal Fiber Component; Alpha-Crystallin B Chain; Epididymis Secretory Protein Li 101; Heat-Shock 20 KD Like-Protein; Crystallin, Alpha B; Alpha(B)-Crystallin; HEL-S-101; CTRCT16; CMD1II; CTPP2; HspB5; MFM2
Background:	UniProt Entry: P02511;NCBI Gene Entry: 1410
Application Information	Molecular Weight: Predicted, 20 kDa, observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6070 Spcies Reactivity: Rat Applctations Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CRYAB
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-crystallin alpha B antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:2,000. Green, isotype control; red, crystallin alpha B.

Immunocytochemical staining of HT-1080 cells with crystallin alpha B antibody 1:1,000. Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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