Aliases:	CTCF; CCCTC-Binding Factor; CFAP108; FAP108; CCCTC-Binding Factor (Zinc Finger Protein); 11 Zinc Finger Transcriptional Repressor; Transcriptional Repressor CTCF; 11-Zinc Finger Protein; CTCFL Paralog; MRD21
Background:	UniProt Entry: P49711;NCBI Gene Entry: 10664
Application Information	Molecular Weight: Predicted, 83 kDa, observed, 140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3520 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CTCF
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of CTCF expression in C2C12 cells using CTCF antibody 1:2,000. Green, isotype control; red, CTCF.

Immunocytochemical staining of C2C12 cells with CTCF antibody 1:1,000. Nuclei were stained blue with DAPI; CTCF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CTCF antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-CTCF antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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