Aliases:	CUL1; Cullin 1; Cullin-1; CUL-1
Background:	UniProt Entry: Q13616;NCBI Gene Entry: 8454
Application Information	Molecular Weight: Predicted, 90 kDa, observed, 80 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1555 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cullin 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Cullin 1 expression in HepG2 cells using Cullin 1 antibody 1:2,000. Green, isotype control; red, Cullin 1.

Immunocytochemical staining of HT-1080 cells with Cullin 1 antibody 1:1,000. Nuclei were stained blue with DAPI; Cullin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cullin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Cullin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.