Aliases:	EIF4EBP1; Eukaryotic Translation Initiation Factor 4E Binding Protein 1; PHAS-I; 4E-BP1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; EIF4E-Binding Protein 1; 4EBP1; BP-1
Background:	UniProt Entry: Q13541;NCBI Gene Entry: 1978
Application Information	Molecular Weight: Predicted, 13 kDa, observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1985 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human eIF4EBP1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of EIF4EBP1 expression in HAP-1 cells using EIF4EBP1 antibody 1:2,000. Green, isotype control; red, EIF4EBP1.

Immunocytochemical staining of HAP-1 cells with EIF4EBP1 antibody 1:1,000. Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-EIF4EBP1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-EIF4EBP1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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