Aliases:	FOXO4; Forkhead Box O4; AFX1; MLLT7; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila); Translocated To, 7 2 3; Fork Head Domain Transcription Factor AFX1; Forkhead Box Protein O4; AFX; Myeloid/Lymphoid Or Mixed-Lineage Leukemia (Trithorax (Drosophila) Homolog); Translocated To, 7
Background:	UniProt Entry: P98177;NCBI Gene Entry: 4303
Application Information	Molecular Weight: Predicted, 54 kDa, observed, 23,65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1470 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human FoxO4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Forkhead box O4 expression in HeLa cells using Forkhead box O4 antibody 1:2,000. Green, isotype control; red, Forkhead box O4.

Immunocytochemical staining of HeLa cells with Forkhead box O4 antibody 1:1,000. Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Forkhead box O4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Forkhead box O4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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