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[KD-Validated] Anti-Huntingtin Rabbit Monoclonal Antibody
[KD-Validated] Anti-Huntingtin Rabbit Monoclonal Antibody
HTT; Huntingtin; IT15; HD; Huntington Disease Protein; Huntingtin (Huntington Disease); HD Protein; LOMARS
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Regular members: $544.0
  • NO.:KD-10482
    Gene name:HTT
    React Species:Human; Mouse; Rat
    Applications:WB; FCM; IHC
    UniProt Entry:P42858
    NCBI Gene Entry:3064
  • Goods click count:249
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Aliases: HTT; Huntingtin; IT15; HD; Huntington Disease Protein; Huntingtin (Huntington Disease); HD Protein; LOMARS
Background: UniProt Entry: P42858;NCBI Gene Entry: 3064
Application InformationMolecular Weight: Predicted, 348 kDa, observed, 348 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6285 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Huntingtin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of Huntingtin expression in HepG2 cells using Huntingtin antibody 1:2,000. Green, isotype control; red, Huntingtin.
Immunocytochemical staining of HepG2 cells with anti-Huntingtin antibody 1:1,000. Nuclei were stained blue with DAPI;Huntingtin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Huntingtin antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-Huntingtin antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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