Aliases:	JUN; Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; V-Jun Avian Sarcoma Virus 17 Oncogene Homolog; AP-1; Transcription Factor AP-1 Subunit Jun; Transcription Factor Jun; Proto-Oncogene C-Jun; Activator Protein 1; Jun Oncogene; C-Jun; AP1; P39; V-Jun Sarcoma Virus 17; Oncogene Homolog; Jun Activation Domain Binding Protein; Enhancer-Binding Protein AP1; Transcription Factor AP-1; Proto-Oncogene CJun; C-JUN; CJUN
Background:	UniProt Entry: P05412;NCBI Gene Entry: 3725
Application Information	Molecular Weight: Predicted, 36 kDa, observed, 39 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2435 Spcies Reaceivity: Human, Mouse Applicseions Tsted: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human c-Jun
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of c-Jun expression in HT-1080 cells using c-Jun antibody 1:2,000. Green, isotype control; red, c-Jun.

Immunocytochemical staining of HT-1080 cells with C-Jun antibody 1:1,000. Nuclei were stained blue with DAPI; C-Jun was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-c-Jun antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-c-Jun antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. JUN, Jun proto-oncogene, AP-1 transcription factor subunit.

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