Aliases:	KRT4; Keratin 4; CYK4; K4; Keratin, Type II Cytoskeletal 4; CK4; Type-II Keratin Kb4; Cytokeratin 4; CK-4; Keratin 4, Type II; Cytokeratin-4; Keratin-4; WSN1
Background:	UniProt Entry: P19013;NCBI Gene Entry: 3851
Application Information	Molecular Weight: Predicted, 56 kDa, observed, 57 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6150 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cytokeratin 4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Keratin 4 expression in H9c2 cells using Keratin 4 antibody 1:2,000. Green, isotype control; red, Keratin 4.

Immunocytochemical staining of H9C2 cells with anti-Keratin 4 antibody 1:1,000. Nuclei were stained blue with DAPI; Keratin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Keratin 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Keratin 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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