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[KD-Validated] Anti-Galectin 3 Rabbit Monoclonal Antibody
[KD-Validated] Anti-Galectin 3 Rabbit Monoclonal Antibody
LGALS3; Galectin 3; GALIG; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein; IgE-Binding Protein; 35 KDa Lectin; Lectin L-29; Galectin
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  • NO.:KD-10550
    Gene name:LGALS3
    React Species:Human; Mouse; Rat
    Applications:WB; FCM; IHC
    UniProt Entry:P17931
    NCBI Gene Entry:3958
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Aliases: LGALS3; Galectin 3; GALIG; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein; IgE-Binding Protein; 35 KDa Lectin; Lectin L-29; Galectin-3; LGALS2; MAC-2; GALBP; MAC2; Epididymis Secretory Sperm Binding Protein; Galactoside-Binding Protein; MAC-2 Antigen; Mac-2; Antigen; CBP 35; CBP35; Gal-3; GAL3; L-31; L31
Background: UniProt Entry: P17931;NCBI Gene Entry: 3958
Application InformationMolecular Weight: Predicted, 26 kDa, observed, 30 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2130 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Galectin 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of Galectin 3 expression in HeLa cells using Galectin 3 antibody 1:2,000. Green, isotype control; red, Galectin 3.
Immunocytochemical staining of HeLa cells with Galectin 3 antibody 1:1,000. Nuclei were stained blue with DAPI; Galectin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Galectin 3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-Galectin 3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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