Aliases:	Mixed Lineage Kinase Domain-Like Protein; FLJ34389; HMLKL; Mixed Lineage Kinase Domain-Like
Background:	UniProt Entry: Q8NB16;NCBI Gene Entry: 197259
Application Information	Molecular Weight: Predicted, 54 kDa, observed, 54 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2935 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MLKL
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of MLKL expression in HepG2 cells using MLKL antibody 1:2,000. Green, isotype control; red, MLKL.

Immunocytochemical staining of HepG2 cells with MLKL antibody 1:1,000. Nuclei were stained blue with DAPI; MLKL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-MLKL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. MLKL, mixed lineage kinase domain like pseudokinase.

Western blotting analysis using anti-MLKL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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