Aliases:	NAD(P) Dependent Steroid Dehydrogenase-Like; SDR31E1; XAP104; H105e3; Sterol-4-Alpha-Carboxylate 3-Dehydrogenase, Decarboxylating; Short Chain Dehydrogenase/Reductase Family 31E, Member 1; Protein H105e3; EC 1.1.1.170; NAD(P) Dependent Steroid Dehydrogenase-Like Protein Transcript; Epididymis Secretory Sperm Binding Protein; H105E3
Background:	UniProt Entry: Q15738;NCBI Gene Entry: 50814
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 38 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB485 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NSDHL
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NSDHL expression in HeLa cells using NSDHL antibody 1:2,000. Green, isotype control; red, NSDHL.

Immunocytochemical staining of HeLa cells with anti-NSDHL antibody 1:1,000. Nuclei were stained blue with DAPI; NSDHL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NSDHL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NSDHL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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