Aliases:	Prohibitin 1; PHB; Prohibitin; Epididymis Secretory Sperm Binding Protein Li 54e; Epididymis Luminal Protein 215; HEL-S-54e; HEL-215
Background:	UniProt Entry: P35232;NCBI Gene Entry: 5245
Application Information	Molecular Weight: Predicted, 30 kDa, observed, 30 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6045 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Prohibitin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-prohibitin antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Western blotting analysis using anti-prohibitin antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:1,000. Nuclei were stained blue with DAPI; Prohibitin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Flow cytometric analysis of prohibitin expression in HT-1080 cells using prohibitin antibody 1:2,000. Green, isotype control; red, prohibitin.

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