Aliases:	RAD50; RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; DNA Repair Protein RAD50; RAD50-2; HRad50; HRAD50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); EC 3.6.3.27; EC 3.6.1.15; EC 3.6.-.-; EC 3.6.3; RAD502; NBSLD
Background:	UniProt Entry: Q92878;NCBI Gene Entry: 10111
Application Information	Molecular Weight: Predicted, 154 kDa, observed, 154 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4115 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Rad50
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RAD50 expression in HeLa cells using RAD50 antibody 1:2,000. Green, isotype control; red, RAD50.

Immunocytochemical staining of HeLa cells with RAD50 antibody 1:1,000. Nuclei were stained blue with DAPI; RAD50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-RAD50 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-RAD50 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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