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[KD-Validated] Anti-ROCK2 Rabbit Monoclonal Antibody
[KD-Validated] Anti-ROCK2 Rabbit Monoclonal Antibody
ROCK2; Rho Associated Coiled-Coil Containing Protein Kinase 2; Rho-Associated, Coiled-Coil-Containing Protein Kinase II; Rho-Associated Protein Kinase 2; P164 ROCK-2; EC 2.7.11.1; ROCK-II; Rho-Associated, Coiled-Coil-Containing Protein Kinase 2; Rho Kinas
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  • NO.:KD-10790
    Gene name:ROCK2
    React Species:Human; Mouse; Rat
    Applications:WB; FCM; IHC
    UniProt Entry:O75116
    NCBI Gene Entry:9475
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Aliases: ROCK2; Rho Associated Coiled-Coil Containing Protein Kinase 2; Rho-Associated, Coiled-Coil-Containing Protein Kinase II; Rho-Associated Protein Kinase 2; P164 ROCK-2; EC 2.7.11.1; ROCK-II; Rho-Associated, Coiled-Coil-Containing Protein Kinase 2; Rho Kinase 2; EC 2.7.11; KIAA0619
Background: UniProt Entry: O75116;NCBI Gene Entry: 9475
Application InformationMolecular Weight: Predicted, 161 kDa, observed, 161 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2030 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ROCK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of ROCK2 expression in HepG2 cells using ROCK2 antibody 1:2,000. Green, isotype control; red, ROCK2.
Immunocytochemical staining of HepG2 cells with ROCK2 antibody 1:1,000. Nuclei were stained blue with DAPI; ROCK2 cochaperone 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ROCK2 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-ROCK2 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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