Aliases:	Ribonucleotide Reductase Catalytic Subunit M1; Ribonucleoside-Diphosphate Reductase Large Subunit; Ribonucleoside-Diphosphate Reductase Subunit M1; Ribonucleotide Reductase M1 Polypeptide; EC 1.17.4.1; RR1; Ribonucleotide Reductase Large Subunit; Ribonucleotide Reductase, R1 Subunit; RIR1; R1
Background:	UniProt Entry: P23921;NCBI Gene Entry: 6240
Application Information	Molecular Weight: Predicted, 90 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB960 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human beta RRM1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RRM1 expression in HepG2 cells using RRM1 antibody 1:2,000. Green, isotype control; red, RRM1.

Immunocytochemical staining of HepG2 cells with RRM1 antibody 1:1,000. Nuclei were stained blue with DAPI; RRM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-RRM1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-RRM1 antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

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