Aliases:	SMAD2; SMAD Family Member 2; JV18-1; MADR2; MADH2; Mothers Against Decapentaplegic Homolog 2; MAD Homolog 2; HMAD-2; HSMAD2; MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2 (Drosophila); SMAD, Mothers Against DPP Homolog 2; Sma- And Mad-Related Protein 2; Mothers Against DPP Homolog 2; Mother Against DPP Homolog 2; Mad-Related Protein 2; SMAD 2; CHTD8; Smad2; JV18; LDS6
Background:	UniProt Entry: Q15796;NCBI Gene Entry: 4087
Application Information	Molecular Weight: Predicted, 52 kDa, observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4150 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Smad2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-Smad2 (S250) expression in C2C12 cells using Phospho-Smad2 (S250) antibody 1:2,000. Green, isotype control; red, Phospho-Smad2 (S250).

Immunocytochemical staining of C2C12 cells with Phospho-Smad2 (S250) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-Smad2 (S250) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-Smad2 (S250) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-Smad2 (S250) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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