Aliases:	STAT1; Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; STAT91; ISGF-3; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; CANDF7; IMD31A; IMD31B; IMD31C
Background:	UniProt Entry: P42224;NCBI Gene Entry: 6772
Application Information	Molecular Weight: Predicted, 87 kDa, observed, 87 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4190 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-STAT1 (S727)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-STAT1 (S727) expression in HepG2 cells using Phospho-STAT1 (S727) antibody 1:2,000. Green, isotype control; red, Phospho-STAT1 (S727).

Immunocytochemical staining of HepG2 cells with Phospho-STAT1 (S727) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-STAT1 (S727) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-STAT1 (S727) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-STAT1 (S727) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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