Aliases:	TIMELESS; Timeless Circadian Regulator; HTIM; TIM1; TIM; Timeless Circadian Clock 1; Protein Timeless Homolog; Timeless (Drosophila) Homolog; Timeless Homolog (Drosophila); Tof1 Homolog (S. Cerevisiae); Timeless Homolog; Tof1 Homolog; TIMELESS1; FASPS4
Background:	UniProt Entry: Q9UNS1;NCBI Gene Entry: 8914
Application Information	Molecular Weight: Predicted, 139 kDa, observed, 150 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2665 Spcies Reaceivity: Human, Mouse Applicseions Tsted: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Timeless
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using anti-Timeless circadian regulator antibody 1:2,000. Green, isotype control; red, Timeless circadian regulator.

Immunocytochemical staining of HepG2 cells with anti-Timeless circadian regulator antibody 1:1,000. Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Timeless circadian regulator antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-timeless circadian regulator antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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