Aliases:	UBE2L3; Ubiquitin Conjugating Enzyme E2 L3; UBCH7; Ubiquitin-Conjugating Enzyme E2 L3; E2 Ubiquitin-Conjugating Enzyme L3; Ubiquitin-Conjugating Enzyme E2-F1; Ubiquitin Carrier Protein L3; Ubiquitin-Protein Ligase L3; L-UBC; Ubiquitin-Conjugating Enzyme E2L 3; Ubiquitin Conjugating Enzyme E2L 3; Ubiquitin-Conjugating Enzyme UBCH7; EC 2.3.2.23; EC 6.3.2.19; E2-F1; UbcM4; UBCE7; UbcH7
Background:	UniProt Entry: P68036;NCBI Gene Entry: 7332
Application Information	Molecular Weight: Predicted, 18 kDa, observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1875 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UBE2L3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of UBE2L3 expression in HepG2 cells using anti-UBE2L3 antibody 1:2,000. Green, isotype control; red, UBE2L3.

Immunocytochemical staining of HepG2 cells with anti-UBE2L3 antibody 1:1,000. Nuclei were stained blue with DAPI; UBE2L3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-UBE2L3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-UBE2L3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .