Aliases:	TOP2A; DNA Topoisomerase II Alpha 2; TOP2alpha; TOPIIA; TOP2; Topoisomerase (DNA) II Alpha 170kDa; DNA Topoisomerase II, Alpha Isozyme; DNA Topoisomerase 2-Alpha; DNA Topoisomerase (ATP-Hydrolyzing); DNA Topoisomerase II, 170 KD; EC 5.99.1.3; DNA Gyrase; EC 5.6.2.2; TP2A
Background:	UniProt Entry: P11388;NCBI Gene Entry: 7153
Application Information	Molecular Weight: Predicted, 174 kDa, observed, 174 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1915 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Topoisomerase II alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of DNA topoisomerase II alpha expression in HepG2 cells using anti-DNA topoisomerase II alpha antibody 1:2,000. Green, isotype control; red, DNA topoisomerase II alpha.

Immunocytochemical staining of HepG2 cells with anti-DNA topoisomerase II alpha antibody 1:1,000. Nuclei were stained blue with DAPI; DNA topoisomerase II alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-DNA topoisomerase II alpha antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-DNA topoisomerase II alpha antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .