Aliases:	UCHL1; Ubiquitin C-Terminal Hydrolase L1; PGP9.5; UCHL-1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; UCH-L1; PARK5; Epididymis Secretory Protein Li 53; Epididymis Luminal Protein 117; EC 3.4.19.12; HEL-S-53; HEL-117; SPG79A; NDGOA; PGP95; SPG79
Background:	UniProt Entry: P09936;NCBI Gene Entry: 7345
Application Information	Molecular Weight: Predicted, 25 kDa, observed, 25 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2125 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PGP9.5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody 1:2,000. Green, isotype control; red, UCHL1.

Immunocytochemical staining of HAP-1 cells with UCHL1 antibody 1:1,000. Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-UCHL1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. UCHL1, ubiquitin C-terminal hydrolase L1.

Western blotting analysis using anti-UCHL1 antibody 1:2,500 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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