Aliases:	UFC1; Ubiquitin-Fold Modifier Conjugating Enzyme 1; HSPC155; Ubiquitin-Fold Modifier-Conjugating Enzyme 1; Ufm1-Conjugating Enzyme 1; NEDSG
Background:	UniProt Entry: Q9Y3C8;NCBI Gene Entry: 51506
Application Information	Molecular Weight: Predicted, 19 kDa, observed, 19 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4245 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UFC1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of UFC1 expression in HeLa cells using UFC1 antibody 1:2,000. Green, isotype control; red, UFC1.

Immunocytochemical staining of HeLa cells with UFC1 antibody 1:1,000. Nuclei were stained blue with DAPI; UFC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-UFC1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-UFC1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.