Aliases:	VPS35; VPS35 Retromer Complex Component; MEM3; PARK17; Vacuolar Protein Sorting-Associated Protein 35; Maternal-Embryonic 3; FLJ10752; HVPS35; Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae); Vacuolar Protein Sorting 35 Homolog; VPS35, Retromer Complex Component; Vesicle Protein Sorting 35
Background:	UniProt Entry: Q96QK1;NCBI Gene Entry: 55737
Application Information	Molecular Weight: Predicted, 92 kDa, observed, 77 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4260 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human VPS35
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of VPS35 expression in C2C12 cells using VPS35 antibody 1:2,000. Green, isotype control; red, VPS35.

Immunocytochemical staining of C2C12 cells with VPS35 antibody 1:1,000. Nuclei were stained blue with DAPI; VPS35 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-VPS35 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-VPS35 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.