Aliases:	WNT5A; Wnt Family Member 5A; HWNT5A; Wingless-Type MMTV Integration Site Family, Member 5A; WNT-5A Protein; Protein Wnt-5a; Epididymis Secretory Sperm Binding Protein
Background:	UniProt Entry: P41221;NCBI Gene Entry: 7474
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2400 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Wnt5a
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Wnt family member 5A expression in HepG2 cells using Wnt family member 5A antibody 1:2,000. Green, isotype control; red, Wnt family member 5A.

Immunocytochemical staining of HepG2 cells with Wnt family member 5A antibody 1:1,000. Nuclei were stained blue with DAPI; Wnt family member 5A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Wnt family member 5A antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Wnt family member 5A antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.