Aliases:	TPM1; Tropomyosin 1; C15orf13; Cardiomyopathy, Hypertrophic 3; Tropomyosin Alpha-1 Chain; CMH3; TMSA; Chromosome 15 Open Reading Frame 13; Epididymis Secretory Protein Li 265; Sarcomeric Tropomyosin Kappa; Tropomyosin 1 (Alpha); Alpha-Tropomyosin; Tropomyosin-1; HEL-S-265; HTM-Alpha; CMD1Y; LVNC9
Background:	UniProt Entry: P09493;NCBI Gene Entry: 7168
Application Information	Molecular Weight: Predicted, 33 kDa, observed, 36 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2545 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Tropomyosin 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Tropomyosin 1 expression in HepG2 cells using anti-Tropomyosin 1 antibody 1:2,000. Green, isotype control; red, Tropomyosin 1.

Immunocytochemical staining of HepG2 cells with anti-Tropomyosin 1 antibody 1:1,000. Nuclei were stained blue with DAPI; Tropomyosin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Tropomyosin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Tropomyosin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .