Summary:

Purpose

Our Quick Step Feline Interleukin 1 Beta (IL-1β)ELISA Kit is to assay IL-1β levels in Feline serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1β. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-1β is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1β and HRP conjugated IL-1β antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1β. You can calculate the concentration of IL-1β in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

The standard curve of QS0077Fe is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.043	0.0405
		0.038
S1	90	1.954	1.9615
		1.969
S2	60	1.318	1.3305
		1.343
S3	30	0.683	0.6910
		0.699
S4	15	0.391	0.3865
		0.382
S5	7.5	0.194	0.1910
		0.188

 

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)