Summary:

 

Purpose

Our Quick Step Mouse Doublecortin Like Kinase 1 (DCLK1)ELISA Kit is to assay DCLK1 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to DCLK1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for DCLK1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain DCLK1 and HRP conjugated DCLK1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DCLK1. You can calculate the concentration of DCLK1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QSD1052Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.036	0.0385
		0.041
S1	600ng/L	1.987	1.9820
		1.977
S2	400ng/L	1.374	1.3780
		1.382
S3	200ng/L	0.733	0.7290
		0.725
S4	100ng/L	0.386	0.3795
		0.373
S5	50ng/L	0.172	0.1800
		0.188

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