Drug Names

Generic Name：Canine rabies virus antibody (RV-Ab) ELISA Kit

Purpose

Our Canine rabies virus antibody(RV-Ab) ELISA kit is to assay RV-Ab levels in Canine

serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided

in this kit has been pre-coated with an antigen specific to RV-Ab. Standards or samples are

added to the appropriate Microelisa stripplate wells and combined to the specific antigen.

Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for RV is added to each

Microelisa stripplate well and incubated. Free components are washed away. The TMB

substrate solution is added to each well. Only those wells that contain RV-Ab and HRP

conjugated RV antigen will appear blue in color and then turn yellow after the addition of the

stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of

450 nm. The presence of RV-Ab is determined by comparing with the CUTOFF value.

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