Determination of Significance：

The mevalonate pathway is a very important metabolic pathway involved in the synthesis of precursors of many important terpenoids and is an important pathway in terpenoid biosynthesis. Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of acetyl coenzyme A and acetoacetyl coenzyme A to produce hydroxymethylglutaryl coenzyme A, a key step in the biosynthesis of cholesterol and isoprenoids.

Measurement Principle：

Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of hydroxymethylglutaryl coenzyme A from acetyl coenzyme A and acetoacetyl coenzyme A. It also produces CoASH, which converts DTNB to TNB (yellow), with characteristic absorbance values at 412 nm.

Self Provided：

Spectrophotometer/ microplate Reader, low temperature centrifuge, adjustable transferpettor, water bath/ constant temperature incubator, micro glass cuvettes/96 well plates, mortar/homogenizer/cell ultrasonicator , ice and distilled water.

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