Product overview

Plant Potato Virus M (PVM) ELISA Kit (Catalog No. SL0052PI) is an enzyme-linked immunosorbent assay kit for detection of the target in plant tissues.

Assay principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to PVM, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for PVM is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain PVM and HRP conjugated PVM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of PVM is determined by comparing with the CUTOFF value. 3

Specifications

• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• User manual (1)
• Closure plate membrane (2)
• Sealed bags (1)
• Microelisa stripplate (1)
• Negative control (0.5ml×1 bottle)
• Positive control (0.5ml×1 bottle)
• HRP-Conjugate reagent (6ml×1 bottle)
• Sample diluent (6ml×1 bottle)
• Chromogen Solution A (6ml×1 bottle)
• Chromogen Solution B (6ml×1 bottle)
• Stop Solution (6ml×1 bottle)
• wash solution

Sample type

plant tissues.

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)