Product overview

Chloramphenicol ELISA Kit (Catalog No. SL0054Ot) is an enzyme-linked immunosorbent assay kit for detection of the target in serum, plasma and cell culture supernatant.

Assay principle

This kit uses a direct competitive ELISA method in microplate coated with Chloramphenicol coupled antigen, adding Chloramphenicol standard or samples, free Chloramphenicol and pore strips Pre-coated Chloramphenicol conjugated antigen compete against Chloramphenicol antibody enzyme marker color with TMB substrate, stop solution added after the color from blue to yellow, with a microplate reader detection wavelength at 45 0 nm, the sample absorbance value and Chloramphenicol content is inversely proportional to the sample through the standard curve Chloramphenicol content. 3. Kit composed (1) Pre-coated Chloramphenicol coupled antigen removable microtiter plates: 1 block (12wells×8strips) (2) Chloramphenicol Standard: 6 bottles ( 1ml / vial), contents were: 0ppb, 0.05ppb,0.15ppb, 0.45ppb,1.35ppb, 4.05ppb. Anti- Chloramphenicol antibody conjugate: 1 bottle (6ml) (3) Color liquid A: 1 bottle (6ml) (4) Color liquid B: 1 bottle (6ml) (5) Stop Solution: 1 bottle (6ml), 2M sulfuric acid. (6) Sample Diluent: 1 bottle ( 10 × , 6ml), used for sample dilution. (7) washing liquid concentrate: 1 b

Specifications

• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)