Summary:

 

Purpose

Our Quick Step Canine Brucella ELISA Kit is to assay the qualitative determination of Brucella in Canine serum, plasma, culture media or any biological fluid.

 

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antibody specific to Brucella, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Brucella is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Brucella and HRP conjugated Brucella antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Brucella is determined by comparing with the CUT OFF value.

Determine the result
Test effectiveness: the average value of positive control ≥1.00; The average value of negative control ≤0.10. 
The critical value (CUT OFF) calculation: critical value = the average value of   negative control + 0.15
Negative judgement:if the OD value<CUT OFF, the sample is Canine Brucella negative.
Positive judgement: if the OD value ≥CUT OFF,the sample is Canine Brucella positive.

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