Summary:

 

Purpose

 

Our Quick Step Rat lipid peroxlde,LPO Elisa Kit is to assay LPO levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an 抗体QS0002Ra specific to LPO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific 抗体QS0002Ra. Then a Horseradish Peroxidase (HRP)- conjugated 抗体QS0002Ra specific for LPO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain LPO and HRP conjugated LPO 抗体QS0002Ra will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of LPO. You can calculate the concentration of LPO in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0002Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μmol/L	0.040	0.0450
		0.050
S1	4.8μmol/L	1.958	1.9555
		1.953
S2	3.2μmol/L	1.308	1.3090
		1.310
S3	1.6μmol/L	0.698	0.7045
		0.711
S4	0.8μmol/L	0.397	0.3935
		0.390
S5	0.4μmol/L	0.209	0.2075
		0.206

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)