Summary:

 

Purpose

 

Our Quick Step Mouse glutamic acid decarboxylase autoantibody,GAD-Ab ELISA Kit is to assay GAD-Ab levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to GAD-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for GAD-Ab is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GAD-Ab and HRP conjugated GAD-Ab antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GAD-Ab. You can calculate the concentration of GAD-Ab in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0240Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.059	0.0575
		0.056
S1	15ng/ml	1.967	1.9730
		1.979
S2	10ng/ml	1.385	1.3895
		1.394
S3	5ng/ml	0.706	0.7110
		0.716
S4	2.5ng/ml	0.382	0.3865
		0.391
S5	1.25ng/ml	0.187	0.1920
		0.197

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