Summary:

 

Purpose

 

Our Quick Step Rat Superoxide Dismutase,sod ELISA Kit (Enzyme Activity) is to assay sod levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to sod. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for sod is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain sod and HRP conjugated sod antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of sod. You can calculate the concentration of sod in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1341Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0U/ml	0.059	0.0575
		0.056
S1	15U/ml	1.967	1.9730
		1.979
S2	10U/ml	1.385	1.3895
		1.394
S3	5U/ml	0.706	0.7110
		0.716
S4	2.5U/ml	0.382	0.3865
		0.391
S5	1.25U/ml	0.187	0.1920
		0.197

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