Summary:

 

Purpose

 

Our Quick Step Rat Immunoglobulin G3, IgG3 ELISA Kit is to assay IgG3 levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to IgG3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for IgG3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IgG3 and HRP conjugated IgG3 antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IgG3. You can calculate the concentration of IgG3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1682Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ug/ml	0.048	0.0465
		0.045
S1	18ug/ml	1.973	1.9760
		1.979
S2	12ug/ml	1.363	1.3675
		1.372
S3	6ug/ml	0.725	0.7220
		0.719
S4	3ug/ml	0.388	0.3860
		0.384
S5	1.5ug/ml	0.187	0.1895
		0.192

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