Summary:

 

Purpose

 

Our Quick Step Sheep β-Hydroxybutyrate (β-HBDH)elisa Kit is to assay β-HBDH levels in Sheepβ-Hydroxybutyrate serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to β-HBDH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for β-HBDH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain β-HBDH and HRP conjugated β-HBDH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of β-HBDH. You can calculate the concentration of β-HBDH in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QSD00163Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μmol/L	0.048	0.0465
		0.045
S1	18μmol/L	1.973	1.9760
		1.979
S2	12μmol/L	1.363	1.3675
		1.372
S3	6μmol/L	0.725	0.7220
		0.719
S4	3μmol/L	0.388	0.3860
		0.384
S5	1.5μmol/L	0.187	0.1895
		0.192

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