Summary:

 

Purpose

 

Our Quick Step Porcine apoprotein B100,apo-B100 ELISA Kit is to assay apo-B100 levels in Porcine serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to apo-B100. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for apo-B100 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain apo-B100 and HRP conjugated apo-B100 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of apo-B100. You can calculate the concentration of apo-B100 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0013Po is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.057	0.0550
		0.053
S1	240ng/ml	1.968	1.9635
		1.959
S2	160ng/ml	1.392	1.3385
		1.285
S3	80ng/ml	0.710	0.7190
		0.728
S4	40ng/ml	0.376	0.3855
		0.395
S5	20ng/ml	0.205	0.1990
		0.193

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