Summary:

 

Purpose

 

Our Quick Step Human Vascular adhesion proein-1 (VAP-1)elisa Kit is to assay VAP-1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to VAP-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for VAP-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain VAP-1 and HRP conjugated VAP-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VAP-1. You can calculate the concentration of VAP-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3211Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.037	0.0400
		0.043
S1	3000pg/ml	1.994	1.9910
		1.988
S2	2000pg/ml	1.314	1.3110
		1.308
S3	1000pg/ml	0.714	0.7215
		0.729
S4	500pg/ml	0.398	0.3925
		0.387
S5	250pg/ml	0.200	0.1950
		0.190

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)