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Quick Step Mouse Tissue Inhibitors of Metalloproteinase 1 (TIMP-1)ELISA Kit
Quick Step Mouse Tissue Inhibitors of Metalloproteinase 1 (TIMP-1)ELISA Kit
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  • NO.:QS0532Mo
    Sensitivity:12 pg/ml
    Assay range:33pg/ml - 2000 pg/ml
    Standard:2700 pg/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
  • Goods click count:123
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Details

 

Summary:

 

Purpose

 

Our Quick Step Mouse tissue inhibitors of metalloproteinase 1,TIMP-1 Elisa Kit is to assay TIMP-1 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TIMP-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TIMP-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TIMP-1 and HRP conjugated TIMP-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TIMP-1. You can calculate the concentration of TIMP-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0532Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Blank Well

0pg/ml

0.049

0.0440

0.039

S1

1800pg/ml

1.956

1.9530

1.950

S2

1200pg/ml

1.389

1.3910

1.393

S3

600pg/ml

0.709

0.7135

0.718

S4

300pg/ml

0.390

0.3875

0.385

S5

150pg/ml

0.195

0.2000

0.205 

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