Summary:

 

Purpose

 

Our Quick Step Rat Kinase MM isoenzyme (CK-MM)elisa Kit is to assay CK-MM levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CK-MM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CK-MM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CK-MM and HRP conjugated CK-MM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CK-MM. You can calculate the concentration of CK-MM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1747Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.035	0.0385
		0.042
S1	1200pg/ml	1.973	1.9790
		1.985
S2	800pg/ml	1.327	1.3330
		1.339
S3	400pg/ml	0.705	0.7105
		0.716
S4	200pg/ml	0.393	0.3885
		0.384
S5	100pg/ml	0.186	0.1895
		0.193

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)