Summary:

 

Purpose

 

Our Quick Step Human 25- hydroxy vitamin D (25-OH-D)elisa Kit is to assay 25-OH-D levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 25-OH-D. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 25-OH-D is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 25-OH-D and HRP conjugated 25-OH-D antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 25-OH-D. You can calculate the concentration of 25-OH-D in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1898Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.044	0.0480
		0.052
S1	60ng/ml	1.999	1.9865
		1.974
S2	40ng/ml	1.302	1.3115
		1.321
S3	20ng/ml	0.687	0.6900
		0.693
S4	10ng/ml	0.392	0.3855
		0.379
S5	5ng/ml	0.202	0.2030
		0.204

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