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Human 3-Hydroxy-kynurenine (3-HK)ELISA Kit
Human 3-Hydroxy-kynurenine (3-HK)ELISA Kit
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Regular members: $374.4
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  • NO.:SL4241Hu
    Sensitivity:0.01ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay range:0.1ng/ml-10ng/ml
    Standard:13.5ng/ml
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Drug Names

Generic Name: Human 3-Hydroxy-kynurenine (3-HK) ELISA Kit

Purpose

Our Human 3-Hydroxy-kynurenine (3-HK) ELISA kit is to assay 3-HK levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 3-HK. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for 3-HK is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 3-HK and HRP conjugated 3-HK antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 3-HK. You can calculate the concentration of 3-HK in the samples by comparing the OD of the samples to the standard curve.

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