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Mouse AMP-activated Protein Kinase (AMPK)elisa Kit
Mouse AMP-activated Protein Kinase (AMPK)elisa Kit
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  • NO.:SL1016Mo
    Assay range:3ng/ml-20ng/ml
    Sensitivity:0.5ng/ml
    Standard:27ng/ml
    Sample Volume:10ul
    Wavelength:450nm
  • Goods click count:52
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Drug Names

Generic Name: Mouse AMP-activated Protein Kinase (AMPK) ELISA Kit

Purpose

Our Mouse AMP-activated Protein Kinase (AMPK) ELISA kit is to assay AMPK levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AMPK. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for AMPK is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AMPK and HRP conjugated AMPK antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AMPK. You can calculate the concentration of AMPK in the samples by comparing the OD of the samples to the standard curve.

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