mitochondria is an organelle covered by two membranes in eukaryotic cells. It is an important organelle structure for producing energy in cells and is the main place where cells carry out aerobic respiration. The energy substances in cells—fat, sugar, and some amino acids—are finally oxidized here, and generate ATP through coupled phosphorylation to supply the needs of cell physiological activities. Research on mitochondrial structure and function is usually performed on isolated mitochondria. The Plant Mitochondrial Protein Extraction Kit provides a complete set of reagents, which can extract plant mitochondrial proteins within 1 hour using a simple and fast method. This kit contains a unique formula that effectively dissolves mitochondrial membrane components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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