Plant nuclear protein extraction kit provides a complete set of reagents, suitable for extracting nuclear proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient. The prepared nuclear protein not only has high purity and maintains natural activity, but also has very little cross-contamination. This kit contains a unique formula that can effectively dissolve plant core components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. This kit is composed of optimized reagent formulas and is particularly suitable for downstream protein interaction research experiments, including immunoprecipitation (IP), co-immunoprecipitation (co-IP), pull-down and other experiments. The protein extracted by this kit can also be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, ELISA, transcription activity analysis, Gelshift gel retardation experiment, and enzyme activity determination. This kit uses enzymatic extraction to prepare plant nuclear proteins, which has improved recovery rate, high purity, and maintains natural activity, but it takes a long time. The extraction process of the non-enzymatic extraction kit is simple, convenient, fast, and can be completed within 1 hour, and there is little cross-contamination, but the recovery rate is lower than that of the enzymatic method. If you need a faster extraction kit, you can choose a non-enzymatic extraction kit. If there is no requirement for extraction speed, you can choose an enzymatic extraction kit. Please choose the kit according to actual needs. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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