Transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of diseases. The preparation of membrane protein samples needs to fully consider the downstream applications such as gel analysis and mass spectrometry analysis, so the preparation of membrane protein samples has become an insurmountable challenge. BBproExtra Filamentous Fungi Membrane Protein Extraction Kit is a fast and efficient high-yield membrane protein extraction kit. The Filamentous Fungi Membrane Protein Extraction Kit can extract total membrane proteins from various small filamentous fungi (molds) except large fungi (mushrooms). The optimized lysate formula can fully release membrane proteins extracted from various septate and non-septate mycelial proteins as well as various mycelial metamorphic proteins, and can be used for the preparation of crude purified proteins and the preparation of membrane proteins. The extraction process is simple and convenient. The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: filamentous fungi

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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