Product overview

Human Amylase Alpha 1 (AMY1) ELISA Kit (Catalog No. SL4177Hu) is an enzyme-linked immunosorbent assay kit for detection of the target in Serum, plasma, cell culture supernatants, body fluid and tissue homogenate.

Assay principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AMY1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Humanradish Peroxidase (HRP)-conjugated antibody specific for AMY1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AMY1 and HRP conjugated AMY1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AMY1. You can calculate the concentration of AMY1 in the samples by comparing the OD of the samples to the standard curve.

Specifications

• Assay range: 0.5ng/ml-40ng/ml
• Sensitivity: 0.1ng/ml
• Standard: 54ng/ml
• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• User manual (1)
• Closure plate membrane (2)
• Sealed bags (1)
• Microelisa stripplate (1)
• Standard: 54ng/ml (0.5ml×1 bottle)
• Standard diluent (1.5ml×1 bottle)
• HRP-Conjugate reagent (6ml×1 bottle)
• Sample diluent (6ml×1 bottle)
• Chromogen Solution A (6ml×1 bottle)
• Chromogen Solution B (6ml×1 bottle)
• Stop Solution (6ml×1 bottle)
• wash solution

Sample type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate.

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)