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Bovine Kidney injury molecule 1 (Kim-1)elisa Kit
Bovine Kidney injury molecule 1 (Kim-1)elisa Kit
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  • NO.:SL0301Bo
    Assay range:5pg/ml-400pg/ml
    Sensitivity:1.2pg/ml
    Standard:540pg/ml
    Sample Volume:10ul
    Wavelength:450nm
  • Goods click count:64
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Drug Names

Generic Name: Bovine Kidney injury molecule 1 (Kim-1) ELISA Kit

Purpose

Our Bovine Kidney injury molecule 1 (Kim-1) ELISA kit is to assay Kim-1 levels in Bovine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Kim-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Kim-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Kim-1 and HRP conjugated Kim-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Kim-1. You can calculate the concentration of Kim-1 in the samples by comparing the OD of the samples to the standard curve.

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