Product overview

Rat anti-hepatitis B virus core IgM (HBc-IgM) ELISA Kit (Catalog No. SL1814Ra) is an enzyme-linked immunosorbent assay kit for detection of the target in In serum, plasma, culture media or any biological fluid.

Assay principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to HBc-IgM, make it to solid-phase antigen. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HBc is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain HBc-IgM and HRP conjugated HBc antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HBc-IgM is determined by comparing with the CUTOFF value. 3

Specifications

• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• User manual (1)
• Closure plate membrane (2)
• Sealed bags (1)
• Microplate (1)
• Negative control (0.5ml×1 bottle)
• Positive control (0.5ml×1 bottle)
• HRP-Conjugate reagent (6ml×1 bottle)
• Sample diluent (6ml×1 bottle)
• Chromogen Solution A (6ml×1 bottle)
• Chromogen Solution B (6ml×1 bottle)
• Stop Solution (6ml×1 bottle)
• wash solution

Sample type

In serum, plasma, culture media or any biological fluid.

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)